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KMID : 0351219940260010039
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Korean Journal of Infectious Diseases
1994 Volume.26 No. 1 p.39 ~ p.45
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In Vitro Effect of Interferon-gamma and Granulocyte-Macrophage Colony Stimulating Factor on Peritoneal Macrophages during CAPD
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우준희
송동화/이지윤/한동철/이상구/황승덕/이희박
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Abstract
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Background :
@EN Peritonitis remains the most common cause of CAPD drop out. Reduced activity of peritoneal macrophages (MØ) may be one of the risk factors for peritonitis during CAPD. Interferon- (IFN-γ) and Granulocyte-Macrophage Colony Stimulating Factor
(GM-CSF)have been shown to control M* activity in animal and in vitro experiments. This study was designed to evaluate the effect of IFN-γand GM-CSF on peritoneal M during CAPD.
@ES Methods :
@EN MØwere separated from overnight peritoneal effluent from eleven CAPD patients using modified Ficoll-Hypaque methods. The 1 x 10E6/ml of peritoneal MØ were incubated with 10E4units/ml of IFN-γor 40㎍/ml of GM-CSF. Peritoneal MØ HLA-DR
expression,
IgG FcⅢ receptor(FcγRⅢ) expression, and bacterial phagocytosis were measured before and after incubation with IFN-γor GM-CSF by two color flow cytometry using monoclonal antibodies.
@ES Results:
@EN 1. HLA-DR expression did not change after IFN- but increased after GM-CSF. although statistically insignificant. 2.MØ Fc RⅢ expression did not change after GM-CSF but increased after IFN-γ, although statistically insignificant. 3.
Bacterial
phagocytosis utilizing FITC labeled S. aureus increased after IFN-γ(P<0.05) and increased also after GM-CSF, although statistically insignificant.
@ES Conclusion :
@EN IFN-γand GM-CSF augmented the peritoneal macorphage activity as shown by significant increase in bacterial phagocytosis and by tendency to enhanced expression of HLA-DR, and Fc RIII expression.
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KEYWORD
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